Insect Cell Expression

Produce recombinant proteins via baculovirus-mediated expression in licensed Sf9 (Spodoptera frugiperda) or Tni (Trichoplusia ni) cells. Both cell lines produce proteins that contain a majority of the post-translational modifications found in mammalian cells, making them suitable for complex eukaryotic targets.

The baculovirus expression vector system (BEVS) uses site-specific transposition to produce recombinant virus encoding the protein of interest. A robust plate-based flow cytometry assay determines baculovirus titers prior to infection, ensuring consistent multiplicity of infection (MOI) and reliable, reproducible production. All cultures are mycoplasma-free, high viability, and low passage.

Optimized serum-free shake flask protocols deliver consistent results from 50 mL to 60 L. Expression completes in 4–6 weeks as part of an 8–12 week gene-to-protein workflow.

• Bac-to-bac compatible construct generation
• Bacmid isolation, verification, and high-titer baculovirus stock production
• Plate-based flow cytometry titer determination
• 50 mL shake flask screening to optimize cell line, MOI, and harvest time
• 1–4 L pilot scale to assess yield and purity
• Up to 60 L production scale
• Gene to protein in 8–12 weeks