Mammalian Cell Expression

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Recombinant Protein Production in Mammalian Cell Lines

Produce recombinant proteins and antibodies in mammalian cells with the post-translational modifications critical for proper folding, secretion, and full biological activity. Transient expression available in licensed HEK293 or CHO cells, plus cell line expansion, banking, and expression in client-provided lines.

Transient Expression Platform

Fully licensed HEK and CHO cell lines engineered with EBV nuclear antigen support high-level episomal plasmid amplification via the OriP element, yielding robust protein expression.

Scalable Production, Fast Turnaround

Optimized serum-free shake flask protocols deliver consistent results from 50 mL to 60 L. Expression completes in 2–3 weeks as part of an 8–12-week gene-to-protein workflow.

  • Construct generation and endotoxin-free plasmid DNA isolation
  • 50–100 mL shake flask culture for screening
  • 1–4 L pilot scale to assess yield and purity
  • Up to 60 L production scale
  • Gene to protein in 8–12 weeks