Cryo-EM Structure Determination for Soluble Proteins
We offer unparalleled expertise in solving protein structures with cutting edge cryogenic electron microscopy (cryo-EM).
Our team has a proven track record of handling the most challenging, non-crystallizable proteins, delivering high-resolution three-dimensional maps and structural data that are crucial for accelerating drug discovery. Soluble proteins, including therapeutic enzymes, multi-subunit complexes, and engineered biologics, are among the target classes we solve routinely.
Why Cryo-EM for Soluble Proteins
Cryo-EM overcomes the challenges of traditional structural methods. X-ray crystallography requires proteins to be crystallized, a process that can be difficult and may require extensive modification of a protein's native structure. Cryo-EM provides structural information for samples in their near-native state, without these constraints.
With cryo-EM, structural biologists can easily answer questions regarding the interactions of small molecules with the chosen target and suggest ways to help design and optimize drug candidates, supporting a structure-based drug design approach.
Target Classes We Solve
- Antibody-Antigen Complexes
- Multi-protein Complexes (e.g. Degraders)
- Protein-Nucleic Acid Complexes
- Receptors
- Enzymes
- RNA
- Other Soluble Proteins
X-ray Crystallography for Soluble Proteins
Historically, X-ray crystallography has been the default technique for protein structure determination. For targets that crystallize readily, X-ray crystallography provides high-resolution 3D structures that reveal binding modes and inform medicinal chemistry.
Cryo-EM overcomes the challenges of traditional structural methods for targets that are difficult to work with and hard to crystallize, providing structural information for samples in near-native states. TrueCourse offers both techniques. Whether your target crystallizes or not, our team delivers the high-resolution structural data that accelerates drug discovery.
Our Track Record
3D structures determined by cryo-EM
of protein structures solved at 3.5 Å or better resolution
Initial timeline for our advanced cryo-EM workflows deliver 3D protein structures
