Cryo-TEM Characterization of AAV Capsid Ratios
The empty-to-full AAV capsid ratio is a critical quality attribute for recombinant adeno-associated virus gene therapies. Empty capsids lack the intended genetic payload, while partially filled particles can complicate interpretation and affect the overall characterization of an rAAV formulation.
Direct, Single-Particle AAV Capsid Analysis
This technical note explains how cryo-transmission electron microscopy directly visualizes individual AAV particles in a native, hydrated state. Interior particle density can be used to distinguish empty, partially filled, and full capsids while the same images reveal aggregation, malformed or damaged capsids, unencapsulated DNA, and other sample features that bulk measurements may average together.
What You’ll Learn in the Technical Note
- How cryo-TEM determines empty-to-full AAV capsid ratios
- Which sample requirements and payload sizes are compatible with the assay
- How automated particle detection and classification support consistent analysis
- How cryo-TEM performance compares with analytical ultracentrifugation
Download the technical note to review the cryo-TEM workflow, method-performance data, and practical considerations for AAV capsid characterization.
