Quality Control of AAV Production with Cryo-TEM
Reliable AAV production depends on more than vector yield. Empty or partially filled capsids, damaged particles, aggregation, host-cell debris, and changes in capsid morphology can all affect the quality and consistency of an AAV-based gene therapy.
Cryo-TEM Across the AAV Production Workflow
This white paper examines cryo-TEM as a direct imaging method for AAV production quality control. By preserving viral particles in a near-native, vitrified state, cryo-TEM can distinguish encapsidation states and assess multiple physical attributes from the same dataset. Computational classification can extend the analysis from individual images to particle populations and high-resolution 3D maps.
What You’ll Learn in the White Paper
- How cryo-TEM identifies empty, partially filled, and full AAV capsids
- How imaging reveals capsid integrity, aggregation, free DNA, and sample purity
- How 2D classification supports particle-level analysis
- How 3D reconstruction can inform capsid engineering and structure-based vector design
Download the white paper to explore where cryo-TEM can support AAV process development, scale-up, comparability, and production quality control.
